PRSS1 and infection: For validation, the late trypsin 1 (AAEL013284), trypsin (AAEL010196, AAEL013715), and trypsin 5G1 precursor (AAEL013712) were chosen, and significant differences between dsGFP and dsAaARLP midguts at 7 days post-infection were confirmed (AAEL013284, P-value = 0.01; AAEL010196, P-value = 0.017; AAEL013712, P-value = 0.045; AAEL013715, P-value = 0.024) (Fig. 3D through G).