Using the previous annotated scRNA-seq data of paired CRC liver metastasis, 4,833 macrophage and monocyte cells in the liver were clustered (Figure 7A).32 Kupffer cell (KC) markers, VSIG4 and CSF1R, were found to be highly co-expressed in the top region of the UMAP (Figure S10A). This evidence concerns the gene VSIG4 and colorectal carcinoma.