Previous human scRNA-seq analyses have shown that SPP1+ macrophages represent a larger proportion of macrophages in liver metastasis compared to primary colonic tumors.32 We next profiled the macrophages in our murine models to see whether an enriched SPP1 and FN1 expressing macrophage population was also found in murine hepatic tumors. The gene discussed is SPP1; the disease is colonic neoplasm.