To confirm the finding, TAS CD8+ T cells were also tested in the second model with B16F10 melanoma using an MHC class I tetramer loaded with TRP2 peptide, and similar results were found(Figures S1 I–L).25,26 In summary, tumors growing in the liver resulted in the highest frequency of TAS CD8+ T cells. The gene discussed is CD8A; the disease is melanoma.