CD8A and hepatocellular carcinoma: We recently performed a spatial co-detection by indexing (CODEX) imaging analysis as well as scRNA-seq analysis from liver tumors and adjacent tissue of HCC patients.44 We repeated scGSEA on this HCC scRNA-seq data set with the same approach as our previous scRNA-seq analysis to define pTRT CD8+ T cells in these HCC patients (Figure 3A).