CD8A and neoplasm: On day 14, the frequency of TAS CD8+ T cells was higher in the intrahepatic TIL compared to subcutaneous TIL (p = 0.0021), whereas no significant differences in tumor weights between intrahepatic and subcutaneous CT26 tumors were found at this time point, ruling out the contribution of tumor burden (p = 0.3792) (Figure 1B).