In the current study, we used Xenium, a subcellular-resolution imaging spatial transcriptomic (IST) platform, with a well-characterized transgenic synucleinopathy mouse model which overexpresses wild-type human α-synuclein (hSNCA) under the murine neuronal Thy1 promoter (Line 61, α-syn-tg). This evidence concerns the gene SNCA and synucleinopathy.