TGF-β prevents immature T cells from differentiating into Th1 cells, promoting Foxp3 to encourage the differentiation of naïve CD4+ T cells into Treg cells and regulating TGF-β signaling to mediate the anti-tumor immune function of Treg inhibiting CD8+ T cells in the TME (21, 22). This evidence concerns the gene CD8A and neoplasm.