Previous studies have shown that NF-κB signaling is mediated by RhoA/ROCK signaling and that TGR5 activation can inhibit RhoA/ROCK/NF-κB signaling in mouse mesangial cells and reduce the expression of FN and TGF-β1, thus alleviating renal fibrosis in DKD mice (111, 112). This evidence concerns the gene NFKB1 and renal fibrosis.