In this study, the frequency of CDC25B-specific mutations in the TCGA database in HCC patients was low, and there were insufficient data to support the study of the effect of the mutations on survival prognosis, but the S160C and G426Afs∗11 mutations, the substitution of a serine by a cysteine at position 160 and a glycine at position 426, due to an insertion or a deletion, resulted in changes in the downstream amino acid sequence and cause code-shifting mutations. Here, CDC25B is linked to hepatocellular carcinoma.