ASCL2 and colorectal carcinoma: At the transcriptional level, the YAP1/KLF5 complex has been shown to engage the promoter region of Ascl2, a key Wnt signaling target gene, thereby amplifying the expression of Ascl2 and enhancing the self-renewal potential of CRC progenitor cells.75 Conversely, inhibition of YAP in colon-derived cell lines markedly dampens the signaling outputs of both Notch and Wnt pathways, subsequently diminishing cell proliferation and viability.