Through CRISPR-mediated knockout and subsequent genome-wide screening, they discovered that the ERN1-JNK-Jun pathway plays a critical role in regulating the sensitivity of these cancer cells to MEK inhibitors, suggesting a novel therapeutic target for overcoming resistance.485 Li et al. utilized genome-wide CRISPR gene knockout screening to identify genes involved in the regulation of oxidative stress, crucial in both tissue inflammation and tumorigenesis. The gene discussed is JUN; the disease is cancer.