This highlights the critical role of ctDNA methylation markers in the surveillance and prognostication of CRC.472 Additionally, the methylation of MLH1 on shores, regardless of genetic background, was found to be independent of promoter CpG island hypermethylation and MSI status.473 There is considerable evidence of CpG island hypermethylation contributing significantly to the reduced expression of protocadherin beta 3 (PCDHB3). This evidence concerns the gene MLH1 and colorectal carcinoma.