Gunes et al. utilized CRISPR to upregulate the Klotho gene in colon cancer cell line Caco-2, observing a significant reduction in cell proliferation and a reversal of tumorigenic properties, which supports the pro-apoptotic role of Klotho in tumor cells.496 Chen and colleagues focused on FAPP2, a modulator of the Wnt/β-catenin signaling pathway, highly expressed in colon cancer cells. Here, KL is linked to colonic neoplasm.