Using a bicistronic construct (Fig. 9A) as described above, GPC2.CD28ζ+/− GLUT1 CAR-T cells were co-cultured with neuroblastoma cell lines expressing ~34,000 (NGP-GPC2) or 6800 (SMS-SAN) molecules of GPC2 on the surface. The gene discussed is SLC2A1; the disease is neuroblastoma.