Moreover, intratumoral inoculation of an attenuated uracil auxotroph strain of T. gondii directly into the 4T1 tumor stimulated anti-tumor immunity in mice, resulting in inhibition of tumor growth and metastasis, increased survival of the tumor-bearing mice, and increased secretion of IL-12 and IFN-γ in both the serum and tumor microenvironment [205]. This evidence concerns the gene IFNG and neoplasm.