Immunohistochemical studies showed diffusely positive SOX10 (Figure 3A), Melan-A (Figure 3B), HMB-45 (Figure 3C), preferentially expressed antigen in melanoma (PRAME) (Figure 3D) and patchy S100 (Figure 3E) immunoreactivity and were negative for p63 (Figure 3F) and GATA3, supporting the diagnosis of melanoma. This evidence concerns the gene PMEL and melanoma.