Notably, TRIB3 levels were found to be elevated in prostate cancer patients (Fig. 2D, P < 0.05), to explored the role of TRIB3 in cell cycle control of prostate cancer cells, two stable TRIB3 knockdown (KD) cell lines were generated for both LNCaP and PC3 cells using short hairpin RNA (shRNA) (Fig. 2E, F). Here, TRIB3 is linked to Familial prostate cancer.