Releasing flagellin B (flaB) and bind to lanthanide up-conversion nanoparticles (UCNP). UCNP emits light in the blue region, activating EcN to secrete flaB, and then it attaches to Toll-like receptor 5 present on macrophage membranes, triggering the immune response against tumor cells through the MyD88-dependent signaling pathway. This evidence concerns the gene TLR5 and neoplasm.