Furthermore, scRNA-seq analysis of six treatment-naïve osteosarcoma tumors, combined with a dataset of 22,035 cells from six osteosarcoma tumors, demonstrated that MDSCs were among the most abundant in the immunosuppressive milieu, as evidenced by MDSC hallmark genes (VCAN, CLEC4E, and CSF3R) (57). Here, VCAN is linked to osteosarcoma.