ERBB2 and ductal breast carcinoma in situ: Wide orientation angle was associated with features of aggressive DCIS behaviour including higher nuclear grade (p < 0.001), DCIS heterogeneity (p < 0.02), comedo necrosis (p < 0.02) TNBC subtype (p < 0.001), hormone receptor negativity (p < 0.001), high proliferation status HER2 positivity (p < 0.03) and (high ki67 score; p < 0.001) (Table S2).