Poorly aligned fibres were associated with aggressive DCIS behaviour including large size (p < 0.003), higher nuclear grade (p < 0.001), comedo necrosis (p < 0.001), TNBC subtype (p < 0.001), hormone receptor negativity (p < 0.001), and high proliferation status (high ki67 score; p < 0.003) (Table S2). Here, MKI67 is linked to ductal breast carcinoma in situ.