CALB2 and cancer: To address whether CALB2 overexpression in CAFs affects cancer cells and given the capability of CALB2+ CAFs to secrete inflammatory cytokines, we first indirectly co-cultured human PDAC organoids with CALB2-OE PSCs (as a substitute for CALB2+ CAFs) or OE-NC PSCs (Control) to examine the effects of CAF-derived soluble factors on PDAC organoids (Fig. 2H).