To determine whether the molecular modifications of gene expression triggered by HLA-G are cell-dependent, we selected several genes (NRP1, NCAM1, KCNIP1, GREM1, CDR1 and CLU) found dysregulated in the RCC7 tumor cells and measured their expression levels in the melanoma cell line M8, expressing or not high levels of HLA-G (Fig. 3b, left). This evidence concerns the gene GREM1 and melanoma.