To functionally determine the impact reduced EMI1 expression has in CIN, transient siRNA-based silencing was employed that revealed significant increases in three CIN phenotypes (nuclear areas; micronucleus formation; aberrant chromosome numbers) in four colonic epithelial cell lines—two malignant/transformed cell contexts (male HCT116 and female SW48) and two non-malignant/non-transformed cell contexts (1CT and A1309). The gene discussed is FBXO5; the disease is cervical squamous intraepithelial neoplasia.