FBXO5 and cervical squamous intraepithelial neoplasia: Generating novel EMI1 knockout models was essential to assess the long-term impact reduced EMI1 expression has on CIN and early disease development, and although EMI1+/−1 and EMI1+/−2 exhibit similar EMI1 protein abundance (~40%), QuantIM analyses revealed both clones exhibited distinct, heterogeneous CIN phenotypes.