The elevated NOS2/CD8 and COX2/CD8 relationships were validated in the Gene Expression Omnibus database inclusive for ER− breast cancer subtype, which showed significant HRs of 5.67 and 3.34 at 5-year survival, for high versus low NOS2/CD8 and COX2/CD8 ratios, respectively, dichotomized at the median (Fig. 2B). This evidence concerns the gene NOS2 and breast cancer.