In our study, we examined the effects of Trolox in AD-induced activated glial cells, which showed higher expressions of Iba1 (Ionized calcium-binding adaptor molecule 1, a marker of activated microglia) and GFAP, (glial fibrillary acidic protein, a marker of activated astrocytes) in the AD-induced mice cortex and hippocampus as compared to saline-treated normal mice. This evidence concerns the gene GFAP and Alzheimer disease.