We also observed a high expression level of ionized calcium-binding adaptor protein 1(Iba1), glial fibrillary acidic protein (GFAP), and other inflammatory mediators and cytokines (phosphorylated nuclear factor kappa B, interleukin 1β and Toll-like receptor 4) in AD-induced mice brains. This evidence concerns the gene TLR4 and Alzheimer disease.