In vivo, transfer of Helios−Tregs, either isolated from Helios reporter mice or from Ikzf2 knockdown mice, were equal to Helios+Tregs in their ability to suppress development of inflammatory bowel disease, but they were inferior in inhibiting the autoimmune disease that follows from transfer of effector T cells from Scurfy mice lacking functional Foxp3 into Rag−/− recipients [41, 42, 48]. Here, IKZF2 is linked to autoimmune disease.