MTA2 and Alzheimer disease: pTau staining was highly consistent between each of the staining runs, with the expected regional differences in levels of pTau staining for each disease: MCI cases showed highest pTau staining in the subiculum, followed by CA1, CA2 and temporal cortex; AD cases showed moderate–high pTau staining in all hippocampal regions studied, with highest levels being observed in the subiculum, CA2 and temporal cortex; and PiD showed highest pTau staining in the DG, CA1, subiculum, and temporal cortex.