To further investigate the biological effects of GPR56 on HCC, we stably overexpressed GPR56 in Hep3B cells with low expression levels using lentivirus and stably knocked down GPR56 in MHCC97H and HLF cells with high expression levels using three different shRNAs, selecting the shRNA-2 for further experiments. The gene discussed is ADGRG1; the disease is hepatocellular carcinoma.