To explore the underlying mechanism by which CD2AP regulates GBM cell malignancy, we performed GSEA analysis using the CGGA and TCGA data and found that CD2AP was positively correlated with multiple molecular pathways of malignant tumors (Fig. 4A), including the NF-κB signaling (Fig. 4B, C). Here, NFKB1 is linked to glioblastoma.