In order to investigate the function of UBE2D1 in various HCC cell lines, we established stable UBE2D1-knockdown cell lines in SNU-739 and HCC-LM3 cells through lentiviral transduction with two UBE2D1-specific shRNAs, while control cells were transduced with an empty pLKO.1 vector lentivirus. Here, UBE2D1 is linked to hepatocellular carcinoma.