To explore the functional significance of miR-101 in cDDP and 5Fu resistance in HCC, we constructed SNU-739 and HCC-LM3 cells that stably overexpressed miR-101 and identified the UBE2D1 expression changes by western blotting and qRT-PCR analysis (Fig. 7A, B). The gene discussed is UBE2D1; the disease is hepatocellular carcinoma.