The aim of this study was to further investigate and gain a deeper understanding of the relationship between periodontitis, potential oral fluid biomarkers including mouthrinse aMMP-8, aMMP-9, tMMP-8, tMMP-9, PMN elastase, MPO, TIMP-1, IL-6, Calprotectin, and prediabetes. The gene discussed is TIMP1; the disease is periodontitis.