Immunoblotting results confirmed that shRNA-mediated LOX knockdown in PTEN-deficient GBM cells (e.g., U87 and PTEN-KO SF763 cells) and GSCs (e.g., GSC23 and GSC7-10) upregulated OLFML3 expression (Figure 3C and Supplemental Figure 3, A and B). This evidence concerns the gene PTEN and glioblastoma.