To further refine the lipid gene signature to its necessary components, we determined the minimal number of lipid metabolism related genes predictive of breast cancer outcomes and arrived at four genes based on their RNA-seq expression in R7 and T47D cell lines compared with their isogenic shRON counterparts (65): DHCR7, BDH2, ELOVL5, and ARSK. Here, BDH2 is linked to breast cancer.