In vivo, the eTreg cells expressing the antigen-specific constructs recognized by pathogenic CD8+ T cells were able to protect against diabetes when co-adoptively transferred into immunodeficient NOD.Scid mice at a 1:1 eTreg to CD8+ T cell ratio (Figure 8B) and significantly decreased the severity of insulitis (Figure 8C). Here, CD8A is linked to diabetes mellitus.