Compared to CAR-T cells that did not secrete anti-CD40 antibodies, MSLN3-CD40 CAR-T cells secreted increased levels of cytokines in response to target antigen stimulation and enhanced antitumor activity in a xenograft tumor model established by subcutaneous inoculation of 1 × 107 SKOV-3-luc cells. The gene discussed is CD40; the disease is neoplasm.