CCK-8, plate cloning and wound healing assays revealed that knockdown of WDR5 (Fig. 5A) inhibited the proliferation and migration abilities of CCA cells (Fig. 5B-E, Figure S1O-P), whereas overexpression of WDR5 (Fig. 5H) promoted these effects (Fig. 5I-L, Figure S1Q-R). This evidence concerns the gene WDR5 and cholangiocarcinoma.