The results of the Cell Counting Kit-8 (CCK-8), plate cloning and wound healing assays revealed that MBD2 knockdown significantly suppressed the proliferation and migration of CCA cells (Fig. 2B-E, Figure S1K-L), whereas the overexpression of MBD2 promoted these effects (Fig. 2I-L, Figure S1M-N). This evidence concerns the gene MBD2 and cholangiocarcinoma.