Followed virus-based regulation studies and the tests of mitochondrial function and glioma feature showed that targeted down-regulation of the DNM1L/DRP1-FIS1 axis in GBM cells can effectively inhibit mitophagy, impede the remodeling of MRC, result in a significant reduction in OXPHOS function, thereby exerting inhibitory effects on the progression of HGG. Here, FIS1 is linked to central nervous system cancer.