CASP3 and non-small cell lung carcinoma: In other primary human NSCLC cells (pNSCLC-2 and pNSCLC-3), as well as in immortalized A549 cells, the establishment of stable ORC6 knockdown using the shORC6-2#-expressing lentivirus (see Fig. 4) similarly triggered Caspase-3 activation (Fig. 5G) and increased the count of nuclei displaying positive TUNEL staining (Fig. 5H), supporting apoptosis activation.