We pre-treated EG7 cells (a mouse lymphoma cell line expressing chicken ovalbumin (OVA)) with itaconate derivatives dimethyl itaconate (DI) or 4-octyl itaconate (4OI) (which were cell-permeable) for 16 h, then co-cultured them with OVA-specific CD8+ T cell hybridoma B3Z cells or primary OT-I T cells. This evidence concerns the gene CD8A and lymphoma.