To further address whether NKG2A knockout CAR33-NK cells had improved efficacy against primary patient material, bone marrow cells (BMCs) derived from ten different AML patients with a substantial blast infiltration (>90% CD45dim AML blasts) were co-cultured for 4 h with CAR33-KLRC1ko-NK cells generated from different healthy donors (in total n = 20 different donor preparations, Fig. 5a). Here, KLRC1 is linked to acute myeloid leukemia.