Additionally, in murine cervical cancer cells (U14), colon cancer cells (CT26), and melanoma cells (B16F10), Western Blot analyses were conducted to evaluate the levels of PD-L1 protein after treatment with PCC16 at varying concentrations (0.1, 0.5, 5, 10, and 50 μM). This evidence concerns the gene CD274 and malignant colon neoplasm.