The STZ-Cre+/Trim56+/+ mice and STZ-Cre+/Trim56flox/flox mice were received daily intraperitoneally injections with 0.5 mg/g AICAR or vehicle (PBS) at 16 weeks of age (6 weeks after DKD initiation), and this injection would last for 14 weeks (Fig. S6E), the expression of AMPKα was determined by western blotting (Fig. S6F). The gene discussed is MICE; the disease is diabetic kidney disease.