TNF and Hyperglycemia: Besides, we observed that silence of PVT1 in podocytes inhibited the expression of NF-κB p-p65, and alleviated the increase of proinflammatory cytokines IL-6, TNF-α and CXCL10 in HG-treated podocytes(Fig. 3I), consistently with the results of Immunofluorescence, showing that inhibition of PVT1 alleviated hyperglycemia-induced nuclear translocation of the p65 subunit of NF-κB (Fig. 3J).