The STZ-Cre+/Trim56+/+ mice and STZ-Cre+/Trim56flox/flox mice were received daily intraperitoneally injections with 0.5 mg/g AICAR or vehicle (PBS) at 16 weeks of age (6 weeks after DKD initiation), and this injection would last for 14 weeks (Fig. S6E), the expression of AMPKα was determined by western blotting (Fig. S6F). This evidence concerns the gene TRIM56 and diabetic kidney disease.