To explore whether PVT1 upregulation is associated with mitochondria damage and increased inflammatory response in DKD, we deleted Pvt1 in kidney podocytes by crossbreeding Nphs2-Cre and Pvt1flox/flox mice to generate Nphs2-Cre/Pvt1flox/flox mice (Cre+/Pvt1flox/flox mice) (Figure S2A), which was identified by tail genotyping and RNA-FISH (Fig. S2B and S2C). The gene discussed is NPHS2; the disease is diabetic kidney disease.