AKT1 and neoplasm: Tumor tissue lysates were prepared, and subsequent assays showed a marked reduction in both mRNA and protein levels of Gαi3 in the aav-shGαi3-s1-treated xenograft tissues (Fig. 9E, F), accompanied by a significant decrease in phosphorylation of Akt (Ser-473) and S6 (Fig. 9G).