Conversely, ectopic overexpression of Gαi3 led to an increase in the phosphorylation of Akt (Ser-473) and S6 in priPC-1 cells (Fig. 6D), highlighting Gαi3’s critical role in Akt-mTOR pathway activation in the pancreatic cancer cells. The gene discussed is AKT1; the disease is pancreatic neoplasm.