Key proteins of autophagy metabolism and their phosphorylation sites, including mTOR, p-mTOR Ser2448, p70S6K, p-p70S6K Thr389, ULK1, p-ULK1 Ser555, BECN1, ATG3, ATG5, ATG7, ATG12 and ATG16L1, were detected to elucidate the specific molecular mechanisms by which AdipoRon inhibits thyroid cancer cell function. This evidence concerns the gene ATG7 and thyroid cancer.