Key proteins of autophagy metabolism and their phosphorylation sites, including mTOR, p-mTOR Ser2448, p70S6K, p-p70S6K Thr389, ULK1, p-ULK1 Ser555, BECN1, ATG3, ATG5, ATG7, ATG12 and ATG16L1, were detected to elucidate the specific molecular mechanisms by which AdipoRon inhibits thyroid cancer cell function. The gene discussed is ATG3; the disease is thyroid cancer.