To understand whether the INs were connected with the presence of specific immune cell subtypes potentially linked to distinct tumour immune escape mechanisms, we analysed the contribution of T-cell and myeloid subsets, including PD-1+CD8 T-cells, exhausted CD8 T-cells expressing PD-1 together with additional immune checkpoints (eg, Tim-3, Lag-3) (CD8 TEX), tissue-resident memory T-cells (TRM), regulatory T-cells (Treg), CD204+and PD-L1+M2 like macrophages and APCs (online supplemental figure 1). This evidence concerns the gene LAG3 and neoplasm.