Additionally, our study found that Imidazole lactate levels, Glutarylcarnitine (C5-DC) levels, and 1-oleoyl-2-linoleoyl-GPE (18:1/18:2) levels mediate the promoting effect of CD39+ resting Treg cells on CRC, while Glutarylcarnitine (C5-DC) levels and 1-stearoyl-2-arachidonoyl-GPE (18:0/20:4) levels attenuate the promoting effect of CD28 on secreting Treg cells. The gene discussed is CD28; the disease is colorectal carcinoma.