15 Inhibition of mice sarcoma growth was achieved using alower concentration of the M. tb enzyme when compared to the requiredconcentration of E. colil-asparaginase.16 Furthermore, it was observedthat M. tb and M. phleil-asparaginaseshave high asparaginase activity, but undetectable glutaminase activity.17 In the present study, the gene encoding the l-asparaginase from M. smegmatis(MsA=Uniprot A0QX50) was cloned and expressed in E. coli BL21 (DE3). The gene discussed is ASPG; the disease is sarcoma.