Given that cucurbitacin I is an inhibitor of p-STAT3 in cancer therapy, we performed RNA-Seq analysis using HBV-infected HepG2-NTCP cells treated with cucurbitacin I. Interestingly, RNA-Seq analysis showed that cucurbitacin I displayed a wide range of biological functions in liver cancer, in which cucurbitacin I was associated with viral diseases. Here, STAT3 is linked to viral infectious disease.