Here, we also detected the protein levels of the TGFβ-Smad signaling pathway (TGFβR2, p-Smad2, and p-Smad3) and myocardial fibrosis markers (Col1α1, Col1, α-SMA) in fibroblasts after 10 ng/mL TGFβ plus miR-194-3p mimic, miR-194-3p inhibitor and TGFβR2 siRNA treatment. This evidence concerns the gene ACTA1 and Myocardial fibrosis.