To investigate the effects of Smad4 on chemokine secretion in hepatocytes, we detected the expression of CCL9, CCL17, CCL20, CXCL5, CXCL9 and CXCL10 in liver tissues from Smad4fl/fl and AlbSmad4-/- HCC mice by RT-qPCR. This evidence concerns the gene SMAD4 and hepatocellular carcinoma.