As shown in Fig.3D, RIPK1 was immunoprecipitated from extracts of HT29 (RIPK3 KO) cells reconstituted with EV as well as RIPK3 (ΔRHIM) cells after viral infection, despite the absence of RIPK3 or the inability to participate in RHIM-dependent interactions, demonstrating that recruitment of RIPK1 to ZBP1 is independent of RIPK3. This evidence concerns the gene RIPK1 and viral infectious disease.