Activation markers of ZBP1-mediated necroptosis, including RIPK3 phosphorylation (pRIPK3) and MLKL phosphorylation (pMLKL), were attenuated in R1-ICR-3-treated HT29-hZBP1 cells during HSV-1(ICP6mut) infection (Fig.3B). The gene discussed is MLKL; the disease is infection.