To this end, we further tested, through the allergy molecular diagnostic, the occurrence and levels of IgE to Der p 1, Der p 2, and Der p 23 in the same population to test the hypothesis that the relationship of the molecular spreading of the IgE responses to Phleum pratense is – in our population sample – inversely related to the molecular spreading of the IgE response to D.pt. Here, IGHE is linked to allergic disease.