To this end, we infected the human in vitro BBB model at a MOI of 1 with the two RVFV strains MRU25010-30 and Mayotte 2008, and at 2 dpi, performed immunostaining of TJ protein ZO-1 on large fields of the hBBB in RVFV and mock-infection conditions (Fig. 3A). Here, TJP1 is linked to infection.