Additionally, it was found that the miR-200 family members directly regulate PD-L1, as demonstrated by the inhibition of luciferase reporter activity when miR-200b or -200c pre-miRs were co-transfected into murine (344SQ) or human (H157 or H1299) lung cancer cells after transfection of wild-type (WT) PD-L1 3′-UTR reporter construct. The gene discussed is CD274; the disease is lung cancer.