25 selected compounds (Supplementary Table S1) were then experimentally evaluated by assessing their effect at 10 μM on the formation of 53BP1 foci by High Throughput Microscopy (HTM), in human osteosarcoma U2OS cells exposed to 10 Gy of ionizing radiation (IR) (Fig. 1A). Here, TP53BP1 is linked to osteosarcoma.