rAd5-16/E6E7Wt with or without the anti-PD-1 checkpoint inhibitor induced a significant increase in the number of CD4+ and CD8+ T cells in the tumor mass along with an increase in Treg cells (defined as CD45 + CD3 + CD4 + FoxP3+) (Figure 3C–E). Here, PDCD1 is linked to neoplasm.